从肺肠上皮组织细胞变化分析肺与大肠相表里的内涵

摘要

Objective:To study and compare the human embryonic cell morphology characteristics,biological characteristics of prolifer-ation and apoptosis of pulmonary and intestinal epithelial in different period of the growth,with a view to the lung and the large intestine tissue on the homology of illustration,and can provide a reference for the theory of traditional Chinese medicine in the lung and the large intestine relationship.Methods:Lung and intestinal tissues in different stages of the embryonic development were embedded in paraffin, HE staining,to observe the epithelial tissue and cell morphology;proliferation and apoptosis of human embryo lung and intestinal epithe-lial cells in different stages were detected by flow cytometry.Results:In the early embryonic stage (9 to 16 weeks),lung and intestinal epithelial tissue and cell morphology were consistent,no significant differences in the biological characteristics of epithelial cell prolifera-tion and apoptosis (P>0.05).In medium embryonic stage (17 to 23 weeks),and late embryonic stage (24 weeks until born)epitheli-al tissue and cell morphology was not consistent,and there were significant differences in the biological characteristics of epithelial cell proliferation,apoptosis (P<0.05).Conclusion:Early embryonic stage (9 to 16 weeks)can provide a basis for"lung"and"the jeju-num,ileum,colon"homology.In addition,the adult lung and intestine is mainly mutual affiliate between functions,but may be related to the original homology of the associated.%目的：研究并比较人胚胎发育不同时期肺与肠上皮细胞形态特征，增殖与凋亡生物学特性，以期能为肺与大肠组织发生上的同源性提供例证，并能为中医理论中肺与大肠相互关系提供参考。方法：胚胎发育不同时期肺与肠组织石蜡包埋，HE染色，观察上皮组织及细胞形态；流式细胞术检测人胚胎不同时期肺与肠上皮细胞的增殖与凋亡，并进行分析。结果：胚胎早期（9～16周龄），肺与肠在上皮组织及细胞形态一致，上皮细胞增殖、凋亡的生物学特性无统计学意义（P＞0.05）。胚胎中期（17～23周）、胚胎晚期（24周～出生）肺与肠在上皮组织及细胞形态不一致，上皮细胞增殖、凋亡的生物学特性有统计学意义（P＜0.05）。结论：胚胎早期（9～16周龄）可以为“肺”与“空肠、回肠、结肠”同源发生提供一定的依据。另外，“肺与大肠相表里”的成体肺肠相关虽主要是功能之间的相互联属，但可能与其原始的同源性相关。