黄芩苷对人肺腺癌 LTEP-A2细胞的抑制作用及机制研究

摘要

目的：研究观察黄芩苷对人肺腺癌LTEP-A2细胞株体外的抑制作用及相关机制。方法：采用不同浓度的黄芩苷干预人肺腺癌LTEP-A2细胞株，用CCK-8方法检测不同时间点不同浓度的黄芩苷对肺癌细胞增殖的抑制程度，接着用细胞划痕试验和Transwell小室侵袭试验观察黄芩苷对肺癌细胞体外迁移、侵袭能力的影响；用Western blot 进一步检测黄芩苷干预后肺癌细胞MMP-2、MMP-9、TGF-β的表达的变化。结果：黄芩苷能明显抑制肺癌细胞增殖；能不同程度降低肺癌细胞的迁移、侵袭、体外划痕愈合能力，呈浓度依赖性；黄芩苷能不同程度下调MMP-2、MMP-9表达，其中以高浓度效果明显，中浓度居中，低浓度作用稍弱，与空白对照组比较，组间差异有统计学意义，P＜0.05；对肺癌细胞TGF-β的表达没有显著影响，与对照组比较，P＞0.05。结论：黄芩苷能不同程度地降低肺癌增殖迁移侵袭的能力，其机制可能是通过下调MMP-2、MMP-9的表达从而对肺癌细胞的增殖、迁移、侵袭发挥抑制作用。%Objective:To explore the inhibition effects and mechanism of baicalin on LTEP-A2 cells in vitro of lung cancer patients . Methods:Human pneumonicadenocarcinoma cell line LTEP-A2 was cultured with different dose of baicalin for different periods of time . Cell proliferation was assayed by using Cell Counting Kit-8, and further in vitro study including tumor cell migration and invasion , wound healing assays were performed .Besides ,MMP-2,MMP-9,TGF-β,which were related to the migration and metastasis potential in lung cancer cell lines were detected by western blot .Results:Baicalin inhibited the proliferation of LTEP-A2 cells in a time and concentration-dependent manner .In tumor cell migration and invasion test , wound healing assays etc .showed significantly decreased migration , inva-sion in baicalin-treated cell groups vs control tumor cells .Further study showed that MMPs decreased after baicalin-treated.The higher doses of baicalin, the lower level of MMP-2/MMP-9.However, baicalin did not have a significant effect on the TGF-β.Conclusion:Ba-icalin inhibits the proliferation and migration of lung cancer LTEP-A2 cells, possibly by decreasing the expression of MMP-2, MMP-9 in part.