东北铁线莲不同提取部位的抗炎镇痛作用研究

摘要

Objective:To study the anti-inflammatory and analgesic activities of different fractions extracted from Clematis manshu-rica Rupr.Methods:The parts of petroleum ether ,chloroform,ethyl acetate and n-butanol were obtained by abstracting ,extracting and concentrating.The experiment was divided into three groups :negative control group ,positive control group and experimental group.In the study,mouse acetic acid writhing and lipopolysaccharide (LPS) induced RAW264.7 cell model were used to evaluate analgesic and anti-inflammatory activity of the four fractions.Results:In the experiment of analgesic ,the inhibition rate of writhing was the most significant in the ethyl acetate fraction.The results of anti-inflammatory experiment suggested that the ethyl acetate fraction showed significant inhibitory effect on nitric oxide (NO).Conclusion:The anti-inflammatory and analgesic effects of differ-ent parts of Clematis mandshurica were different ,and ethyl acetate fraction showed the most significant anti-inflammatory and anal-gesic activity.%目的:研究和比较东北铁线莲不同提取部位的抗炎镇痛活性.方法:东北铁线莲经提取、萃取、浓缩得到石油醚部位、氯仿部位、乙酸乙酯部位和正丁醇部位,实验分为阴性对照组、阳性对照组和实验组,分别利用小鼠醋酸扭体疼痛模型和脂多糖诱导RAW264.7细胞炎性反应模型评估4个提取部位的镇痛活性和抗炎活性.结果:在镇痛实验中,乙酸乙酯部位对小鼠扭体的抑制率最高;抗炎实验结果显示,乙酸乙酯部位抑制NO的效果最为显著.结论:东北铁线莲不同部位的抗炎镇痛效果不同,其中乙酸乙酯部位抗炎镇痛活性最强.