目的:观察活血膏对骨性关节炎模型大鼠运动能力的干预效应,同时评估其对关节软骨超微结构以及Wnt/β－cate－nin信号通路标志性蛋白表达的影响.方法:将30只SD大鼠随机分为空白组、模型组以及活血膏组,每组10只.其中模型组及活血膏组大鼠接受4%木瓜蛋白酶关节腔注射以复制骨性关节炎模型,随后活血膏组大鼠接受活血膏外用涂抹,连续干预14 d,比较3组大鼠关节腔软骨下骨组织形态学、软骨形态以及软骨组织Wnt－2、β－catenin含量的变化.结果:1)接受造模的大鼠膝关节活动度明显低于空白组,其中活血膏组大鼠膝关节活动度大于模型组,差异均有统计学意义(P＜0.05). 2)模型组大鼠骨小梁出现明显硬化现象,局部存在断裂,骨小梁排列顺序紊乱,可见骨赘形成,活血膏组大鼠骨小梁排列明显较模型组整齐,断裂现象明显减少.接受造模大鼠BS／BV较空白组升高,Tb.Th及Tb.N均较空白组下降,其中活血膏组较模型组改善,差异均有统计学意义(P＜0.05);3)HE染色显示空白组大鼠软骨面形态正常,基质着色均匀正常,潮线清晰可见;模型组大鼠软骨出现不同程度的纤维化,软骨细胞数量减少,潮线模糊不清;活血膏组软骨细胞较模型组增多,可见部分潮线. 4)Western Blot检测法提示模型组软骨组织Wnt－2、β－catenin含量较空白组明显升高,活血膏组虽较空白组上调但较模型组明显降低,差异均有统计学意义(P＜0.05).结论:活血膏对骨性关节炎大鼠的软骨及软骨下骨有保护效应,其作用机制可能与抑制Wnt／β－catenin信号通路表达有关.%Objective:To observe the intervention effect of Huoxue ointment on the motor function of osteoarthritis rats , and to e－valuate its effect on the ultrastructure of articular cartilage and the expression of Wnt /β－catenin signaling pathway.Methods:A to－tal of 30 SD rats were randomly divided into blank group , model group and Huoxue ointment group , with 10 rats in each group. The model group and Huoxue plaster group were treated with intra－articular injection of 4% papain to replicate the osteoarthritis model, then Huoxue ointment group were treated with external application of Huoxue ointment , intervention for 14 consecutive days.Rat articular subchondral bone tissue morphology , cartilage morphology and cartilage tissue Wnt－2, beta－catenin of three groups were compared.Results:1) The activity of the knee joint in the model rats was significantly lower than that in the blank group, and the motion range of the knee joints in Huoxue ointment group was greater than that in the model group .The difference was statistically significant (P＜0.05).2) The trabecular bone showed obvious hardening phenomenon in the model group .There were local fractures.The trabecular arrangement was disorder.The formation of osteophyte was observed.The bone trabecular ar－rangement in Huoxue ointment group was significantly more than that in the model group , and the fracture phenomenon was signifi－cantly reduced.BS/BV of rats with model establishment elevated significantly compared with control group , and Tb.Th and Tb.N were lower than that in blank group.The huoxue ointment group improved compared with the model group , and the differences were statistically significant (P＜0.05).3) HE staining showed that the rats in cartilage matrix of the blank group had normal morphology, and uniform coloring was normal with visible and clear tide line.The cartilage in model group showed different de－grees of fibrosis, and the number of cartilage cells was decreased.The tidal line was blurred; Chondrocytes of Huoxue ointment group increased in comparison with the model group , and part of the tidal line was visible.4) Western Blot test showed that the Wnt－2 and β－catenin levels of cartilage tissue in model group were significantly higher than those in blank group , while Huoxue ointment group was higher than that in blank group.It was significantly lower than that in model group , and the difference was sta－ tistically significant (P＜0.05).Conclusion:Huoxue ointment can protect cartilage and subchondral bone in osteoarthritis rats , and its mechanism may be related to inhibiting the expression of Wnt /β－catenin signaling pathway.
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