参芪止血胶囊对大鼠增生型子宫内膜Bcl-2,Bax表达的影响

摘要

Objective: To investigate the effects of ShenQi ZhiXue capsule(SQZC) on expression levels of Bcl-2 and Bax in endometrium of rats for exploring mechanism of SQZC in treating dysfunctional uterine bleeding. Method: Model rats with proliferative endometrium were established through intragastric administration diethylstilbestrol (1 mg/kg) for 10 days. The model rats were randomly assigned into 360, 180, 90 mg/kg of SQZC groups administered for 14 days and model control group. The expression levels Bcl-2 and Bax were detected with immunohistochemical SP method. Result: The Bax expression levels in different dosage of SQZC groups were much higher than that in the model control group(P<0.05), and no statistical meaning when they were compared with normal control group(P>0.05). The expression levels of Bax in the 360, 180, 90 mg/kg of SQZC groups were much lower than that in model control group(P<0.05), there was no significant difference when they were compared with normal control group(P>0.05). Conclusion: SQZC capsule can adjust the expression of Bax and Bcl-2 in endometrium.%目的:观察参芪止血胶囊(SQZC)对大鼠增生型子宫内膜中凋亡调节蛋白Bcl-2和Bax表达的影响,探讨其治疗功能性子宫出血病的作用机制.方法:以乙烯雌酚(1 mg/kg)给大鼠灌胃,连续10天,建立大鼠子宫内膜增生模型.造模后大鼠随机分为模型对照组,参芪止血胶囊高、中、低剂量组(360,180,90mg/kg),连续给药14天.采用免疫组化SP法检测各组大鼠子宫内膜中Bcl-2和Bax的表达水平.结果:SQZC各剂量组子宫内膜中Bax的表达水平均明显高于模型对照组(P<0.05),与正常对照组比较差异无统计学意义(P>0.05);SQZC各剂量组Bcl-2表达水平均明显低于模型对照组(P<0.05),SQZC高、中剂量组与正常对照组比较差异无统计学意义(P>0.05).结论:SQZC对大鼠子宫内膜Bcl-2和Bax的表达有调节作用.