解酒护肝方对不同体质大鼠酒精性肝损伤的保护作用

摘要

Objective:To observe the protective effects of JieJiu HuGan prescription on alcoholic liver injury of rats with different constitutions. Methods: All the rats were randomized into 11 groups: normal group, Yang deficiency model group, medication groups of preventing Yang deficiency (low dose group of warm-nature prescription, high dose group of warm-nature prescription, low dose group of cold-nature prescription and high dose group of cold-nature prescription), Yin deficiency model group, medication groups of preventing Yin deficiency (low dose group of warm-nature prescription, high dose group of warm-nature prescription, low dose group of cold-nature prescription and high dose group of cold-nature prescription), except normal groups, the models of Yang deficiency were established by muscular injection of hydrocortisone (30 mg/kg) and the ones of Yin deficiency by intragastric administration of thyroxine aqueous solution (25 mg/kg). According to the constitutions, the rat models were given with preventive medicine in different dosages (4.15, 0.83 g/kg, intragastric administration), after one hour, the rats were perfused with Erguotou (112 degrees proof, 0.009 mL/g), the levels of AST and ALT in the serum of the rats were detected after perfused for five consecutive weeks. Results:Compared with the normal group, AST, ALT and AST/ALT of the model group and the preventive medication groups were increased, which suggested liver injury in different degrees (P<0.05);AST and ALT were improved significantly in the high dosage group which were medicated against the constitution (P<0.05);AST and ALT were decreased remarkably in the high dosage group which were medicated based on the constitution (P<0.05). Conclusion: JieJiu HuGan prescription based on the constitution could effectively prevent alcoholic liver injury of the rats.%目的：观察解酒护肝方对不同体质大鼠酒精性肝损伤的保护作用。方法：将大鼠随机分为正常组、阳虚模型组、阳虚预防给药组(温低组、温高组、寒低组、寒高组)、阴虚模型组、阴虚预防给药组(寒低组、寒高组、温低组、温高组)等11组，除正常组外，阳虚组采用30 mg/kg肌注氢化可的松，阴虚组采用甲状腺素水溶液25 mg/kg分别建立阳虚模型、阴虚模型，造模完成后针对体质预防给药，分高、低剂量组(按生药量计为4.15,0.83 g/kg)，1小时后灌胃0.009 mL/g 56度红星二锅头，持续灌注5周后测定大鼠血清谷草转氨酶（AST）、谷丙转氨酶（ALT）水平。结果：与正常组比较,模型组与预防给药组的AST、ALT及AST/ALT比值均增高,提示有不同程度的肝损伤(P＜0.05)；违背体质用药的高剂量组血清AST、ALT增高明显(P＜0.05)；符合体质用药的高剂量组血清AST、ALT明显降低(P＜0.05)。结论：针对体质的解酒护肝方能有效预防大鼠酒精性肝损伤。