袋装口含型无烟气烟草制品烟碱体外释放分析

摘要

研制了一套能够模拟口含型无烟气烟草制品烟碱在口腔释放的体外释放装置,并采用该装置对袋装口含型无烟气烟草制品烟碱及游离烟碱的释放进行分析.该装置主要包括溶媒存储瓶、恒流泵、恒温槽、预热线圈、保温管、恒温溶出池和释放液收集装置,其中恒温溶出池用来模拟人体口腔,通过恒流泵、恒温槽、预热线圈、保温管、恒温溶出池使烟碱在模拟条件下释放.该装置操作方便,稳定性好,能够有效地模拟口含型无烟气烟草制品在口腔的释放行为,区分不同产品间的烟碱释放差异,对口含型无烟气烟草制品的质量评价、烟碱调控以及烟碱生物利用度评价具有一定的实用价值.%A device for simulating nicotine release from oral smokeless tobacco product in human mouth was developed, which consisted of a container for solvent, a constant flow pump, a constant temperature trough, a pre-heating coil, a thermostatic tube, a thermostatic cell, and a liquid collector.The thermostatic cell acted as a buccal cavity to ensure nicotine to be released in the cell under a constant temperature of 37 ℃.The device was convenient in operation and good in stability and could effectively simulate nicotine release behavior of oral smokeless tobacco products and distinguish the difference of nicotine release between different products.The novel technique is suitable for quality evaluation, and nicotine level control of oral smokeless tobacco products as well as the assessment of bioavailability of nicotine.