口用型无烟气烟草制品中烟碱的测定

摘要

In order to accurately determine the content of nicotine in oral smokeless tobacco products, a gas chromatography-flame ionization (GC-FID) method was developed, wherein the pretreatment of samples and test conditions were optimized. A total of 23 kinds of pouched snus, 20 kinds of nicotine chewing gums and 2 kinds of dissolvable tobacco were analyzed by the developed method. The results showed that: 1) The limit of detection and limit of quantitation were 0.01 and 0.05 mg/g, respectively; the recoveries were 90.2%-106.2%, and intra-day and inter-day precisions were 0.80%-3.27% and 0.92%-4.81%, respectively. 2) Two samples of pouched snus did not contain nicotine, the nicotine contents in the other pouched snus were 6.19-17.08 mg/g. For those chewing gum samples nicotine levels were 1.20-3.95 mg/g, and those two dissolvable tobacco were 0.85 and 1.62 mg/g, respectively. This method is sensitive, accurate and suitable for the quantitative determination of nicotine in oral smokeless tobacco products.%为准确测定口用型无烟气烟草制品中烟碱的含量(质量分数),通过对样品前处理和检测条件进行优化,建立了测定口用型无烟气烟草制品(袋装口含烟、胶基烟、含化烟)中烟碱的气相色谱-氢火焰离子化法(GC-FID),并测定了23个袋装口含烟、20个胶基烟和2个含化烟样品.结果表明:①该方法的检出限0.01 mg/g,定量限0.05 mg/g;回收率90.2%~106.2%,日内和日间精密度分别为0.80%~3.27%和0.92%~4.81%.②所测定的袋装口含烟样品中有2个不含烟碱,其余21个样品中烟碱的含量为6.19~17.08 mg/g;胶基烟样品中烟碱的含量为1.20~3.95 mg/g,2个含化烟烟碱含量分别为0.85、1.62 mg/g.该方法灵敏度和准确度高,适合于口用型无烟气烟草制品中烟碱的定量检测.