DART-MS/MS法测定烟草及烟草制品中的烟碱

摘要

In order to determine nicotine in tobacco and tobacco products in real time, an online method based on Direct Analysis in Real Time (DART) ionization technique combined with mass spectrometry was established by optimizing parameters such as extraction solvent type, extraction solvent dosage and injection rate. The samples of five commercial cigarettes and five tobacco leaves were analysed by the established method, and the determined values were compared with those obtained using a continuous flow method. The results showed that: 1) Under the optimized conditions (30 mL ultrapure water as extraction solvent and sample injection rate of 0.6 mm/s), the analysis could be completed within 20 s. 2) The linear range of the method was 0.01-2.00 mg/mL with R2 of 0.9991, the recovery ranged from 98.5％ to 102.3％, and the detection limit and the limit of quantification were 6.05 and 20.17 ng/mL. 3) The relative error between DART-MS/MS method and continuous flow method was 0.24％-2.74％ . The established method is rapid, reliable and suitable for the real-time on-line determination of nicotine in tobacco and tobacco products.%为实时在线检测烟草及烟草制品中的烟碱,通过优化萃取溶剂种类、萃取溶剂用量、进样速率等参数,建立了基于实时直接分析(DART)新型原位电离技术和质谱(MS)技术测定烟草及烟草制品中烟碱含量(质量分数)的方法.采用该方法测定了5种成品卷烟及5种烟叶样品,并与连续流动法检测结果进行了对比.结果表明:①用30 mL超纯水萃取、进样速率为0.6 mm/s时效果较好,20 s即可完成样品检测.②方法线性范围为0.01～2.00 mg/mL,R2为0.9991,回收率为98.5％～102.3％,检出限和定量限分别为6.05和20.17 ng/mL.③DART-MS/MS法与连续流动法测定结果相对偏差为0.24％～2.74％.该方法快速、可靠,适用于烟草及烟草制品中烟碱的实时在线检测.