在合成1-脱氧-1-L-脯氨酸-D-果糖(Fru-Pro)和1-脱氧-1-L-丙氨酸-D-果糖(Fru-Ala)的基础上,以Fru-Pro和Fru-Ala作标样,建立了高效液相色谱-质谱联用技术(HPLC/MS)定性定量分析烟草中这2种物质的方法,并采用该方法测定了16个烟草样品.结果表明:①通过保留时间和SCAN模式下的质谱碎片能准确定性这2种物质,通过SIM模式选择正离子(m/z)278.0和252.0可分别定量Fru-Pro和Fru-Ala;②Fru-Pro和Fru-Ala的检出限分别为25.0和30.0μg/g,回收率93.1%和105.8%,RSD 4.23%和3.97%;③3个烤烟烟叶样品中的Fru-Pro和Fru-Ala含量远远高于3个白肋烟叶样品,5个国产烤烟型卷烟样品中这2种物质的含量比1个进口烤烟型卷烟、2个国产和2个进口混合型卷烟样品都高.该方法适合烟草中这2种1-氨基-1-脱氧-2-酮糖的同时定性定量分析.%Using the in-house synthesized N - (1 - deoxy - D - fructos - 1 - yl) -L- proline ( Fru - Pro) and N - (1 - deoxy - D - fructos - 1 - yl) - L-alanine ( Fru - Ala) as standards, a HPLC/MS method for qualitatively and quantitatively analyzing these two compounds in tobacco was developed.Sixteen tobacco samples were analyzed, the results showed that: 1 ) Fru - Pro and Fru - Ala were identifiable via retention time and main mass fragmentation under SCAN mode and quantifiable under selected positive ion mode at ( m/ z) 278.0 and (m/z) 252.0, respectively; 2) The recoveries of Fru - Pro and Fru - Ala were 93.1％ and 105.8％ with the relative standard deviations of 4.23％ and 3.97％ and the limits of detection of 25.0 and 30.0 μg/g, respectively; 3) The contents of Fru - Pro and Fru - Ala in 3 flue-cured tobacco leaf samples were much higher than that in 3 burley tobacco samples, in 5 domestic Virginian-type cigarette samples were higher than that in 1 imported Virginian-type cigarette sample, 2 domestic and 2 imported blended-type cigarette samples.The method was suitable for the determination of these two compounds in tobacco samples.
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