离子色谱法测定卷烟主流烟气中氨含量的方法改进

摘要

In order to determine the ammonia in mainstream cigarette smoke accurately, ion chromatography (IC) method was improved, and the improved method was applied to determine the ammonia in mainstream smoke of 59 cigarettes measured under ISO and Health Canada (HC) smoking regimes, respectively. The results showed that: 1) The samples were collected, extracted, and purified by graphitized carbon blacks (GCB) column, then determined by ion chromatography using Dioncx IonPac CS16 cation exchange column with methane sulfonic acid (MSA) as eluent. 2) The calibration curve of ammonia was linear in the range of 0 to 1 |ig/mL with the correlation coefficient of 0.9992 and the limit of detection of 0.0067 ug/mL. The recoveries ranged from 96% to 104% with the relative standard deviation of 2.8% to 4.5%. 3) The ammonia measured under HC smoking regime was in the range of 173% to 378% relative to that measured under ISO smoking regime. The problems in sample solution stability and pollution to chromatographic system were eliminated.%为准确测定主流烟气中的氨含量,对离子色谱(IC)测定方法进行了改进,并采用该方法测定了59种卷烟在ISO和加拿大卫生部(Health Canada,HC)深度抽吸条件下主流烟气中的氨含量.结果表明:①烟气样品经捕集、萃取和石墨化炭黑柱(GCB)净化后,以甲基磺酸(MSA)为淋洗液,采用CS16阳离子交换柱分离.②在0～1 μg/mL范围内,线性相关系数为0.9992,方法检出限为0.0067 μg/mL,相对标准偏差为2.8％ ～ 4.5％,回收率为96％ ～ 104％.③HC深度抽吸条件下卷烟主流烟气中NH4+含量是ISO抽吸条件下的173％ ～ 378％.改进后的测定方法较好地解决了样品溶液不稳定、色谱系统易被污染的问题.