卷烟烟气总粒相物诱导A549细胞的氧化应激研究

摘要

In order to evaluate the oxidative stress of human lung adenocarcinoma cells (A549 cells) induced by total particulate matter (TPM) in cigarette smoke, A549 cells were exposed to the TPM of mainstream smoke of 3R4F reference cigarette. The dose-response relationship between TPM dose and the viability of A549 cells was tested by the neutral red assay, and two indicators of oxidative stress, intracellular glutathione (GSH) and extracellular superoxide dismutase (EC-SOD), were determined. The results indicated that: 1)A good dose-response relationship existed between TPM dose and the viability of A549 cells. 2) When oxidative stress occurred, the rate of reduced and oxidized state glutathiones (GSH/GSSG)in cells significantly decreased comparing with negative control, it did not change with the increase of exposure duration. EC-SOD did not increase significantly in 4 hours, while it increased significantly after 24-hour exposure. The experimental results provide a reference for the research of cells’oxidative stress induced by cigarette smoke.%为研究卷烟烟气总粒相物（TPM）对人肺腺癌细胞（A549）的氧化损伤，捕集3R4F参比卷烟主流烟气的TPM，对A549细胞进行染毒，采用中性红细胞毒性法测试TPM与A549细胞存活率的剂量-效应关系，检测了细胞内谷胱甘肽（GSH）和细胞外超氧化物歧化酶（EC-SOD）两种氧化应激指标。结果表明：①TPM与A549细胞存活率之间存在良好的剂量-效应关系。②细胞发生氧化应激时，细胞内还原态谷胱甘肽和氧化态谷胱甘肽比值（GSH/GSSG）相对于阴性对照组有显著降低，且不随染毒时间的增加而变化；EC-SOD在染毒4 h后没有显著增加，但24 h后有显著增加。