Skp2和p27kip1在乳腺癌组织中的表达及其相关性的研究

摘要

Objective: To explore the mRNA expression of Skp2 and p27kipl in breast cancer, and their contribution to the earcinogenesis and development of breast cancer. Methods: Semi-quantitative reverse transcription polymeraae chain reaction (RT-PCR) was used to detect in 40 breast cancer tissues, 20 breast fibroadenoma tissues and 20 normal breast tissues to compare and analyze expressions of Skp2 and p27kipl in breast cancer and clinical pathological factors. Results: The average mRNA expression level of Skp2 was significantly higher in breast cancer tissues than that in breast fibroadenoma and normal tissues(P < 0.05), but no significant difference between normal breast and fibroadenoma tissues(P > 0.05). The average mRNA expression level of p27kipl was significantly lower in breast cancer tissues than that in breast fibroadenoma and normal breast tissues(P<0.05), but no significant difference between normal breast tissues and fibroadenoma tissues(P> 0.05). The mRNA expression of Skp2 was positively correlated to the pathological grade and lymphatic metastasis. But the mRNA expression of p27kipl was negatively correlated with the pathological grade and lymphatic metastasia. There were no significant differences between expressions of Skp2 and p27kipl and age, menopausai status, tumor size and the pathological grade. The mRN A expression of Skp2 in breast tissues was negatively correlated to the mRNA expression of p27kip1. Conclusion: Skp2 and p27kipl were involved in the earcinogenesis and development of breast cancer. The joint detection may be helpful for breast cancer prediction and prognosis.%目的:检测乳腺癌组织中Skp2和p27kip1 mRNA的表达,探讨其在乳腺癌发生、发展中的作用及其相关性.方法:采用半定量逆转录聚合酶链反应法( RT-PCR)检测40例乳腺癌组织,20例乳腺纤维腺瘤组织和20例正常乳腺组织中Skp2 mRNA和p27kip1 mRNA的表达,并分析其在乳腺癌组织中的表达水平与临床病理因素的关系.结果:乳腺癌组织中Skp2 mRNA表达较乳腺纤维腺瘤组织及正常乳腺组织明显增高(P＜0.05),在正常乳腺组织和乳腺纤维腺瘤组织中差异无统计学意义(P＞0.05)；而p27kip1 mRNA表达较乳腺纤维腺瘤组织及正常乳腺组织明显降低(P＜ 0.05),在正常乳腺组织和乳腺纤维腺瘤组织中表达差异无统计学意义(P＞0.05).乳腺癌组织中Skp2 mRNA表达水平与组织学分级、淋巴结转移程度呈正相关；p27kip1 mRNA表达水平与组织学分级、淋巴结转移程度呈负相关.2种基因表达均与患者年龄、绝经状况、肿瘤大小及病理学分期无关(P＞0.05).乳腺癌组织中Skp2 mRNA与p27kip1 mRNA表达呈负相关.结论:乳腺癌组织Skp2和p27kip1参与了乳腺癌的发生、发展过程,两指标的联合检测可能有助于预测乳腺癌预后.