目的 研究选择性环氧合酶(COX)-2抑制剂塞来昔布对幽门螺杆菌(Hp)诱导的人永生性胃上皮细胞株(GES-1)转化细胞是否有抑制增殖、诱导凋亡的作用.方法 在体外将幽门螺杆菌悉尼株(SS1)与GES-1细胞共培养建立GES-1细胞转化模型,用四甲基偶氮唑蓝(MTT)法、平板克隆形成实验检测细胞转化情况；再利用MTT法、流式细胞仪检测观察塞来昔布对转化细胞的增殖及凋亡的影响.结果 与GES-1对照细胞相比,Hp(SS1)与GES-1细胞共同培养2个月后细胞增殖活性明显增高.塞来昔布作用于GES-1转化细胞,随着药物浓度的升高(50、75、100μmol/L),GES-1转化细胞增殖活性逐渐降低,凋亡率逐渐升高.结论 幽门螺杆菌长期作用于胃上皮细胞,可以诱导其转化；塞来昔布可抑制GES-1转化细胞的增殖,并促进其凋亡.%Objective To investigate the effect of selective cyclooxygenase-2 (COX-2) inhibitor (celecoxib) on the inhibited proliferation and induced apoptosis in gastric epithelial cell transformation induced by Helicobacter pylori (H.pylori) . Methods In vitro,the immortalized 'normal' gastric epithelial cell line GES-1 was co-cultured for two months with H. pylori Sydney strain (SSI) to establish the GES-1 transformation cell model. The cell transformation was measured by 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and colony formation assay. The proliferation and apoptosis of transformed cells treated by celecoxib were detected by MTT assay and flow cytometry. Results The proliferative activity of GES-1 cells co-cultured with H. pylori (SSI) for two months was significantly higher compared with that of control GES-1 cells. The proliferative activity of GES-1 transformed cells was inhibited with the increased concentration of celecoxib (50,75 and 100 μmol/L),and the apoptotic rate of the GES-1 transformed cells was gradually increased. Conclusion The transformation of gastric epithelial cells can be induced by H. pylori for a long term. Celecoxib inhibits the proliferation and induce apoptosis of GES-1 transformed cells.
展开▼
