c-met在新疆哈萨克族与汉族食管鳞癌组织及血清中的表达及意义

摘要

目的：探讨新疆哈萨克族（哈族）与汉族食管鳞癌患者组织及血清中c-met蛋白的表达及其与临床病理特征的关系。方法收集哈族和汉族食管鳞癌组织及其对应的癌旁食管组织各50例，采用免疫组织化学法检测组织中c-met蛋白的表达；取以上食管鳞癌患者血清标本，另择哈族和汉族健康体检者血清各40例，采用酶联免疫吸附（ELISA）法测定血清中c-met蛋白的含量。结果 c-met蛋白在哈族和汉族食管鳞癌组织中的阳性表达率分别高于其对应的癌旁组织，且c-met蛋白在哈族和汉族食管鳞癌患者血清中的含量均分别高于其对应的健康体检者。食管鳞癌组织和血清中，c-met蛋白表达在不同肿瘤浸润深度、淋巴结转移、TNM分期间差异有统计学意义，其中浸润至深肌层及突破浆膜（T3+T4）高于浸润黏膜及黏膜下层组（T1+T2），有淋巴结转移高于无淋巴结转移，TNM分期ⅡB+Ⅲ期高于Ⅰ+ⅡA期；c-met蛋白表达在不同民族、性别、年龄、组织分化程度间的差异均无统计学意义。结论 c-met蛋白与哈族和汉族食管鳞癌的发生发展、浸润、转移及分期均有关，其在食管鳞癌组织和血清中的高表达，可能预示食管癌易浸润转移，并提示临床分期较晚。%Objective To explore the relationship between serum expression of c-met protein and clinic pathological features in esophageal squamous cell carcinoma (ESCC) of Kazakh and Han people. Methods A total of 50 samples of ESCC and its corresponding adjacent tissues of Kazak and Han patients were collected. The expression of c-met protein was detected by immunohistochemistry assay. The serum c-met protein was detected in 40 Kazak and Han ESCC samples and 40 Kazak and Han controls using ELISA method. Results The positive expression rates of c-met protein were higher in Kazak and Han esophageal squamous cell carcinoma patients than those in its corresponding adjacent tissues respectively. And the serum expression rates of c-met protein were higher in Kazak and Han esophageal squamous cell carcinoma patients than those of healthy controls. There were significant differences in the different tumor invasion, lymph node metastasis and TNM stage of esophageal squamous cell carcinoma tissue and serum between groups, in which the infiltrated deep muscle and se⁃rous breakthrough (T3+T4) were higher than the infiltration of the mucosa and submucosa group (T1+T2), with lymph node metastasis was higher than that without lymph node metastasis, and TNM stage of ⅡB+Ⅲwas higher than that ofⅠ+ⅡA. There was no significant difference in the expression of c-met protein between ethnicity, gender, age and degree of differenti⁃ation. Conclusion The c-met protein expression is related to the occurrence and development, the invasion, metastasis and TNM stage of ESCC in Kazak and Han nationalities. The high expression of c-met protein in tissue and serum may indicate the invasion and metastasis of esophageal cancer and prompt a late clinical stage.