高效液相色谱法测定3种水溶性荧光增白剂

摘要

A new method was investigated to determine three fluorescent whitening agents by liquid chromatography (DAD). The fluorescent whitening agents were analyzed by Eclipse Plus C18 column (4.6 mm ×100 mm, 3.5 μm), employing acetonitrile- water as the mobile phase in a gradient elution mode. Meanwhile, the detection wavelength was 348 nm. The results showed that there was good linear relationship in the range of 5~1 000 mg/L for BA (r=0.999 7); while in the range of 1~1 000 mg/L for CXT (r=0.999 9). BBU also had good linear relationship in the range of 50~1 000 mg/L (r=0.999 9). The RSD values of three fluorescent whitening agents were 2.82%, 1.71% and 1.48% (n=6), respectively. This method was precise and had good reproducibility, accurate and reliable data.%探讨了高效液相色谱（DAD检测器）法测定荧光增白剂BA、CXT、BBU，采用Eclipse Plus C18（4.6 mm×100 mm，3.5μm）色谱柱，流动相乙腈-水进行梯度洗脱，检测波长为348 nm。结果表明，BA在5~1000 mg/L具有良好的线性关系（r=0.9997）， CXT在1~1000 mg/L具有良好的线性关系（r=0.9999），BBU在50~1000 mg/L具有良好的线性关系（r=0.9999）。3种荧光增白剂的RSD值分别为2.82%、1.71%以及1.48%（n=6）。该方法精密度高，重现性好，数据准确可靠。