复合酶对甘蔗渣栽培灵芝菌糠的降解作用

摘要

采用3种工业饲料酶(纤维素酶、漆酶、木聚糖酶)酶解以蔗渣为主要原料的灵芝菌糠，分别测定发酵终产物中总糖含量，还原性糖含量，纤维组分中纤维素、半纤维素、木质素含量。结果表明在总酶活1000 u/g，料液比1：10，酶解时间12 h，纤维素酶：漆酶：木聚糖酶=1：1：1条件下，灵芝菌糠中纤维素含量降解率为36.81%，半纤维素含量降解率为29.09%，木质素含量降解率为17.97%，体现了3种工业饲料酶对甘蔗渣栽培的灵芝菌糠有效的降解作用。%Substrate after cultivation ofGanoderma lucidum with sugarcane bagasse is hydrolyzed by three industrial feed enzymes (cellulase, laccase and xylanase), then the total sugar contents, reducing sugar contents, cellulose contents, hemicellulose contents and lignin contents in the final product of fermentation are determined respectively. The results show that under the condition of total enzyme activity 1000 u/g, material liquid ratio 1:10, enzymolysis time 12 h, cellulase:laccase:xylanase=1:1:1, the three industrial feed enzymes have effective degradation in the contents of cellulose, hemicellulose and lignin with 36.81%, 29.09% and 17.97% compared with substrate after cultivation ofGanoderma lucidum with sugarcane bagasse.