杜鹃精细胞的分离

摘要

The mature pollen of Rhododendron simsii is bicellular, containing one vegetative cell and one germ cell, with the sperm cells forming in pollen tube. Semi vitro technique was used in style pollinated to make pollen tube sprout from style. Then the pollen tube burst by osmotic shock method and released a pair of sperm cells which connected to vegetative nucleus. The sperm cells separated kept activities proved by FDA method. A fairly large number of separated sperm cells could be collected by micromanipulator.%　　杜鹃成熟花粉为二胞型，含一个营养细胞和一个生殖细胞，其精细胞在花粉管中形成。应用半离体技术培养杜鹃已授粉花柱，使花粉管从花柱中长出，再用渗透压冲击法促使花粉管破裂，释放出一对与营养核相连的精细胞。分离的精细胞经FDA方法检测，证明具活性。用显微操作仪可收集数量较多的分离精细胞。