不同诱导子对枇杷悬浮细胞生长及熊果酸、齐墩果酸含量的影响

摘要

在已建立的稳定枇杷细胞悬浮培养系的试验中，研究加入诱导子水杨酸（SA）、茉莉酸甲酯（MJ）、水解酪蛋白（CH）和酵母提取物（YE）对枇杷悬浮细胞生长及次生代谢产物熊果酸（UA）、齐墩果酸（OA）含量的影响。结果表明，从细胞生长、UA及OA含量三方面综合考虑，以MJ为100.0 mg·L-1时培养最佳，此时细胞生长、UA及OA含量分别为对照的1.081、2.540及2.590倍。%Using the suitable loquat cell suspension culture as test materials, the influence of different elicitors (salicylic acid, SA; jasmonic acid, MJ; casein acid hydrolysate, CH; yeast extract, YE) on the cell growth, the accumulation of ursolic acid (UA) and Oleanic acid (OA) were studied. The results showed that the addition of MJ 100.0 mg·L-1was advantageous to loquat cell suspension culture, the fresh weight, UA and OA content, were 1.081, 2.540, 2.590 times to the control, respectively.