有柄石韦总多酚含量测定及其超声提取工艺优化

摘要

To optimize the extraction of total polyphenols in Pyrrosia petiolosa by ultrasonic, the content of the polyphenols was used as investigation index, and the total polyphenol content was determined by Folin-Ciocalteus colorimetric. The extraction process parameters for polyphenols were optimized by the single factor and the orthogonal experiment. The results showed that there was a good linearity between gallic acid concentration and absorbance in the range 1~10 mg·L-1 (y=2.406x-0.012, R= 0.9994);The optimum conditions were as follows: solvent concentration 55%, ultrasound time 60 min, extraction temperature 75℃, solid to liquid ratio 1∶10. The polyphenols extraction rate was 21.47 mg·g-1 in the best technical conditions.%为优选有柄石韦Pyrrosia petiolos中总多酚超声提取工艺，采用Folin-Ciocalteus比色法对总多酚进行含量测定，通过单因素和正交试验，对提取工艺参数进行优化。结果表明，没食子酸对照品在浓度为1～10 mg·L-1范围内，与吸光度呈良好线性关系（y=2.406x-0.012，R=0.9994），有柄石韦总多酚的最佳提取工艺条件为：55%乙醇，料液比1∶10（W/V），超声提取60 min，超声提取温度75℃，总多酚提取率为21.47 mg·g-1。