酿酒酵母ADH2基因沉默表达载体的构建

摘要

It was to synthesize the silencing gene SADH2 of Saccharomyces cerevisiae with overlapping PCR.The silencing gene SADH2 was inserted in pYES2.0 to construct S.cerevisiae ADH2 gene silencing expression vector.Sequencing results showed that the expression vector pSADH2 was correct.%利用重叠延伸PCR获得酿酒酵母基因沉默组件SADH2,经酶切与酿酒酵母穿梭质粒pYES2.0连接,构建酿酒酵母ADH2基因沉默表达载体。经酶切及测序鉴定成功构建了ADH2基因沉默表达载体pSADH2。