酿酒酵母ADH2基因沉默菌株构建的初步研究

摘要

本实验通过构建酿酒酵母ADH2基因沉默表达载体，电转化法转化酿酒酵母工程菌Y01，获得酿酒酵母ADH2基因沉默突变株S01。发酵实验结果表明沉默突变株乙醇产量相对较低。说明沉默突变株体内乙醇合成途径受到干扰。%The main purpose of this research is to construct a ADH2 gene silencing strain S01 by the method of constructing ADH2 gene silencing expression vector than transformed intoSaccharomyces cerevisiae Y01. The results showed that the ethanol production of the mutant strain was relatively low. The alcohol metabolic pathway in this transformant si interfered.