羌活挥发油的GC－MS分析及其抗炎镇痛的药理作用初探

摘要

目的： GC－MS分析羌活挥发油主要成分，并观察其抗炎、镇痛的药理作用。方法通过 GC－MS联用分析羌活挥发油的主要成分；通过二甲苯小鼠耳部肿胀实验观察抗炎作用；通过醋酸扭体法和小鼠热板法观察其镇痛效果。结果鉴定出羌活挥发油17种化合物；羌活挥发油的高、中、低剂量均可显著抑制二甲苯所致小鼠耳肿胀，具有良好的抗炎作用；羌活挥发油高剂量组可显著减少醋酸所致的小鼠扭体次数，镇痛率可达47．15％；但羌活挥发油对热板所致小鼠疼痛无明显抑制作用。结论羌活挥发油的主要成分为（1R）－（＋）－α－蒎烯，松油醇，且总挥发油具有明显的抗炎作用和镇痛作用。%OBJECTIVE To analyze main ingredient of volatile oil of Notopterygium incisum by GC-MS and ob-serve pharmacological effects of anti-inflammatory and analgesic.METHODS GC-MS was employed.The analgesic effect was observed with the response of writhes and hot plate test.The anti-inflammatory effect was observed by mice inflammation models caused by xylene and mice models with reinforce permeability of abdominal cavity capillary caused by acetic acid.RESULTS Seventeen volatile constituents were determined.Volatile of Notopterygium inci-sum could obviously inhibit the xylene induced ear swelling.High dose of volatile had significant analgesic effect on writhing responses induced by acetic acid,47.15%rate of analgesic,but had no effect on analgesic response in the hot plate test.CONCLUSION Main ingredient of volatile oil of Notopterygium incisum are (1R)-(+)-α-pinene, terpineol.The volatile oil have definite effects on anti-inflammation and peripheral analgesia.