狭叶羌活根茎和根的挥发油成分的GC-MS分析

摘要

Aim To analyse the chemical constituents of the essential oil extracted from the rhizome and root of Notopterygium incisum Ting ex H.T. Chang, providing scientific basis for quality control. Methods The total essential oil was extracted by water-steam distillation and separated by capillary gas chromatography (GC). The components were determined by normalization method, and identified by GC-MS. Results GC-MS exhibited 242 peaks and 83 compounds were identified, accounting for 75.77% of the total essential oil. Conclusion In the total essential oil of the rhizome and root of N. incisum, monoterpenes and sesquiterpenes accounted for 13.63% and 67.93%, respectively, in which (1S)-β-pinene (1.67%), 3-carene (1.05%), limonene (1.22%), and 1S-endo-bornyl acetate (1.68%) as the monoterpenes and its derivatives, and (+)-β-elemene (6.78%), sativene (1.54%), α-caryophyllene (2.64%), germacrene D (1.67%), eudesma-4 (14),11-diene (2.36%), α-selinene (2.42%), δ-cadinene (1.55%), 3,7,11-trimethyl-2,6,10-dodecatrien-1-ol (1.03%), (±)-elemol (5.18%), (-)-spathulenol (1.40%), guaiol (3.81%), dehydroxy-isocalamendiol (1.06%), γ-eudesmol (1.05%), α-eudesmol (7.97%), bulnesol (3.09%), and carotol (2.30%) as the sesquiterpenes and its derivatives were main components. In addition, isopropyl trans-cinnamate was the maximum compound (11.3%) of the total essential oil.%目的 分析狭叶羌活(Notopterygium incisum Ting ex H. T. Chang)根茎和根的挥发油的化学成分,为其质量评价提供科学依据.方法 采用水蒸气蒸馏法提取狭叶羌活根茎和根的挥发油,用GC毛细管柱进行分析,归一化法测定其相对含量,并用GC-MS法鉴定化学成分.结果 检出242个色谱峰,初步鉴定出83个化合物,占挥发油总量的75.77%.结论 狭叶羌活根茎和根的挥发油中的化学成分主要为单萜和倍半萜类化合物,两者分别占总油中化学成分的13.63 % 和 67.93 %.(1S)-β-蒎烯 (1.67%)、3-蒈烯 (1.05%)、柠檬烯 (1.22%)和1S-endo-醋酸冰片酯 (1.68%) 为单萜类化合物中的主要成分;(+)-β-榄香烯 (6.78%)、苜蓿烯 (1.54%)、α-石竹烯 (2.64%)、异大香叶烯 D (1.67%)、桉烷-4 (14),11-二烯 (2.36%)、α-芹子烯 (2.42%)、δ-杜松烯 (1.55%)、3,7,11-三甲基-2,6,10-十二烷三烯-1-醇 (1.03%)、(±)-榄香烯 (5.18)、(-)-匙叶桉油烯醇 (1.40%)、愈创醇 (3.81%)、去羟基异菖蒲烯二醇 (1.06%)、γ-桉醇 (1.05%)、α-桉醇 (7.97%)、异愈创木醇 (3.09%)和胡萝卜醇 (2.30%) 为倍半萜类化合物中的主要成分.总挥发油中,反式肉桂酸异丙酯占11.3%,为所有化合物中含量最高者.