吉非替尼与5－氟尿嘧啶、紫杉醇联合对胃癌细胞MKN－45的抑制作用

摘要

目的：探讨吉非替尼与5－氟尿嘧啶（5－FU）、紫杉醇（ Paclitaxel）联合序贯对人胃癌细胞MKN－45的抑制作用，筛选出体外对胃癌有效的联合用药方案。方法采用免疫组化法鉴定MKN－45细胞内EGFR相关抗原，采用MTT法测定5－FU、紫杉醇与吉非替尼联合对 MKN－45细胞的抑制作用。利用combidrug软件分析联合用药的作用效果。结果表明MKN－45细胞内 EGFR相关抗原表达阳性。先用化疗药物24h序贯吉非替尼48h方式给药时，5－氟尿嘧啶，紫杉醇与吉非替尼均有协同作用，CI值＜1；先用吉非替尼48h序贯化疗药物24h方式给药时，5－氟尿嘧啶，与吉非替尼有协同作用，CI值＜1，紫杉醇与吉非替尼在fa＞0．85时，CI值＞1，呈拮抗作用。结论5－氟尿嘧啶与吉非替尼具有协同作用，联合效果不受序贯给药顺序的影响。先用紫杉醇24h序贯吉非替尼48h具有协同作用，联合效果优于其余两种给药方案。%OBJECTIVE To investigate the inhibitory effect of Gefitinib and 5-fluorouracil ( 5-Fu ) , Paclitaxel ( Paclitaxel ) combined with sequential on human gastric cell MKN-45 ,and choose Screening of effective combination regimens on gastric cancer in vitro .METHODS Immunohistochemistry method was used to identify EGFR related antigen in MKN-45 cell,and MTT method was adopted to determine joint inhibitory effects of 5-FU,paclitaxel and ge-fitinib on MKN-45 cells.RESULTS EGFR antigen in the MKN-45 cells appeared positive .First with the chemo-therapy drug 24h sequential gefitinib 48h administration 5-fluorouracil,paclitaxel and gefitinib had synergistic effect , CI0.85.CONCLUSION 5-fluorouracil and gefitinib had synergistic effect ,combined effect is not affected by the sequential order of administra-tion.First with paclitaxel 24 h sequential gefitinib 48 h had a synergistic effect ,Which is better than the other two regi-mens.