目的 研究骨缝来源的间充质细胞的体外成骨潜能及骨形成蛋白(BMP)2转染对其成骨分化的影响.方法 取10d左右的SD乳鼠颅骨矢状缝及缝边缘2mm骨组织,分离培养骨缝间充质细胞.取第三代细胞进行骨向诱导,培养1周后进行碱性磷酸酶(ALP)染色.同时取第三代细胞进行BMP2转染,培养7d和10 d时检测ALP活性.结果 ①骨缝间充质细胞在骨向诱导1周后,可见多数细胞开始呈簇状生长,大部分细胞呈ALP染色阳性；②BMP2转染的实验组与对照组比较,培养7d和10 d ALP活性均明显增加(P＜0.05).结论 该方法所获得的骨缝来源的间充质细胞具有很强的体外增殖活性和良好的成骨能力；BMP2能够诱导骨缝间充质细胞成骨.%Objective To investigate the osteogenic potential of suture-derived mesenchymal cells (SD-MCs) in vitro and the effects of transfected BMP2 on osteogenic differentiation of SD-MCs. Methods SD-MCs derived from the sagittal suture of 10-day-old SD rats were cultured in vitro. The cells of 3rd subculture were osteoblastic-induced and stained with ALP after 7-day culture. The same cells were transfected with BMP2 and ALP staining was applied at the 7th day and 10th day. Results ① After 7-day osteoblastic-induced differentiation,clustered growth of SD-MCs was observed and SD-MCs were positive in ALP staining. ②The ALP activity increased significantly in BMP2 transfected group compared with control group after 7-day and 10-day culture( P <0.05). Conclusions SD-MCs obtained from this method have strong proliferation activity and osteogenic potential. BMP2 could induce the osteogenic differentiation of SD-MCs.
展开▼
