三维打印超顺磁性氧化铁纳米组织工程支架

摘要

Objective To fabricate scaffolds containing superparamagnetic iron oxide nanoparticles( SPIONs) by 3D printing technol⁃ogy in order to make scaffolds with better biocompatibility for tissue engineering. Methods Gelatin⁃alginate hydrogel scaffolds adding SPIONs and not adding SPIONs were both fabricated by 3D printing technology. Stereomicroscope, transmission electron microscope (TEM),vibrating sample magnetometer(VSM)and flow cytometry(FCM)were used to characterize the surface topography, internal structure, magnetic properties and biological properties. Results Stereomicroscope showed that scaffolds with regular structure and u⁃niform pore could be successfully fabricated by 3D printing technology, and TEM showed that SPIONs were distributed evenly in the hydrogel matrix .The VSM results showed that scaffolds containing SPIONs exhibited superparamagnetism. The FCM results showed that compared with the control scaffolds, the cell proliferation index in the scaffolds in experimental group elevated, and the magnetic scaf⁃folds could promote cell proliferation, which indicated that the magnetic ones had better biocompatibility. Conclusion Tissue engi⁃neering scaffolds can be successfully fabricated by 3D printing technology and scaffolds with SPIONs show better compatibility compared with those without SPIONs.%目的：利用先进的三维打印技术，制备含有超顺磁性氧化铁纳米颗粒（ superparamagnetic iron oxide nanoparticles，SPI⁃ONs）的支架，以期获得生物相容性更佳的组织工程支架。方法通过三维打印的方法分别制备添加以及不添加SPIONs的明胶和海藻酸钠水凝胶支架，用体视显微镜、透射电镜、振动样品磁强计以及流式细胞仪对其形貌、磁学性能和生物学性能进行比较。结果体视显微镜显示通过三维打印的方法可以制备出形态规则、孔径均匀的三维支架，透射电镜显示SPIONs在水凝胶基体中均匀分布。振动样品磁强计结果显示添加了SPIONs的支架具有超顺磁性。流式细胞仪检测结果显示实验组支架细胞增殖指数升高，具有促进细胞增殖的作用，表现出更好的生物相容性。结论利用三维打印的方法可以成功制备出含SPIONs的组织工程支架，且该新型支架表现出更加优良的生物相容性。