鲜红斑痣光动力治疗中皮肤光谱特点的变化

摘要

尝试利用漫反射光谱和荧光光谱检测鲜红斑痣皮肤在光动力治疗中的变化特点,用于分析治疗中组织光学特性的变化,指导光剂量的制定.在光动力治疗中,采用微型光纤光谱仪监测PWS皮肤的漫反射光谱和荧光光谱,结合PWS结构特点以及皮肤中主要吸光基团的吸收光谱,分析术中、术后相关组织成分变化及对应的光学特性变化.PDT治疗中PWS皮肤的反射光谱的变化主要是血红蛋白和黑色素吸收波段的反射率增加或降低；部分患者伴有反射光谱形态的变化,主要是两种血红蛋白吸收光谱差异明显的波段.PWS组织的荧光光谱也反映了组织中血液含量的信息.漫反射光谱监测可反映组织中血液含量、血氧含量、黑色素含量变化引起的组织光学特性变化,进一步进行深入研究可提供PDT中组织光学特性动态变化的信息.%To analyze the change of optical property of PWS skin during photodynamic therapy, the reflection spectra and fluorescence spectra of PWS skin during PDT treatment were measured using mini-optical fiber spectrograph. The change of reflection spectra and fluorescence spectra were analyzed according to the histological structure of PWS skin and the absorption spectra of the main chromophores in skin (melanin and hemoglobin). The result showed that the spectra changed significantly at the wavelength at which melanin and hemoglobin have high absorption, or at which obvious difference existed in the absorption between HbO2 and Hb. The monitoring of reflection spectra and fluorescence spectra during PDT can provide information about the dynamic change of the optical property of PWS tissue.