Using laser correlation spectroscopy to study gold nanoparticles diffusion dynamics for photodynamic therapy.

摘要

The dynamic behaviour of gold nanoparticles of different shapes (sphere and rod) were studied under different experimental conditions to be used for drug delivery in photodynamic therapy. Fluorescence correlation spectroscopy (FCS) using single photon excitation had been used to study the diffusion dynamics of gold nanoparticles of different shapes in water solution to utilize these results for further enhancement for the photodynamic therapy. We analyzed four different colloidal gold solutions (colloidal gold nanosphere solution, colloidal gold nanosphere-photosensitizer solution, colloidal gold nanoshpere- Rhodamine B solution, and colloidal gold nanorods-Rhodamine B solution) using the custom software (MAZ-2015) we made to analyze the results for these samples by calculating and fitting the autocorrelation. We found that we could get a reliable correlation when laser power was higher than 4.2 microW. The phosphate buffered saline shows the best results over the nanopure water and culture media when used to dilute the colloidal gold nanoparticles solutions, and the (MAZ-2015) software provides a comparable results to commercial software provided by the microscope vendors. The results indicated that gold nanosphere conjugated with Rhodamine-B can be a potential candidate to study drug transport to cells in photodynamic therapy treatment.;The form and content of this abstract is approved. I recommend its publication. Approved: Approved: Tim C. Lei.