荷叶生物碱的提取及体外抑菌和凝血活性研究

摘要

The experiment was involved in extraction of alkaloid from lotus leaves, its effects of inhibition ability and in vitro coagulation activity on Staphylococcus aureus, Escherichia cob', Candida, AspergiⅡus niger were investigated. The 9% of Lotus leaf alkaloid had strong antibacterial activity, the antibacterial circle diameter size and MIC values were 18.6mm: Escherichia cob, Staphylococcus aureus7%; 16.3mm, 12%;15.6mm9%; Candida. Aspergillus niger 8.5mm, 21%;High, medium and low concentrations lotus leaf alkaloid extract compared with the placebo in vitro shortened coagulation time rate of 1.5%, lower than 30%, the coagulation effect was not significant.%本试验通过提取荷叶生物碱,探讨荷叶生物碱对金黄色葡萄球菌、大肠杆菌、酵母菌、黑曲霉的抑制能力以及体外凝血活性.实验结果表明,荷叶生物碱具有较强的抑菌活性,其浓度为9％的荷叶生物碱抑菌圈直径大小和最小抑菌浓度值分别为:大肠杆菌18.6mm、7％；金黄色葡萄球菌16.3mm、12％；酵母菌15.6mm、9％；黑曲霉8.5mm、21％；高、中、低浓度荷叶生物碱提取液与空白对照相比体外凝血时间缩短1.5％,凝血效果不显著.