新城疫病毒山东分离株F和HN基因克隆与进化分析

摘要

Fusion protein gene (F) and Hemaggutinin-Neuraminidase gene (HN) of fire NDV isolates collected from 2007-2008 were cloned and sequenced. The result showed that F gene length of five NDV isolates was 1662 bp, encoding SS3 amino acids. Five isolate share common cleavage site motif 112R-R-Q-K-R-F117 corresponding to virulent NDV characterization. Comparison of variable region of F gene (47 -420 nt) of 5 isolates with other reference strain showed that 4 chicken isolates belonging to genotype Ⅶd, whereas pigeon isolate belonging to genotype Ⅵ. Homology of F gene nucleotide and deduced amino acid between 5 isolates were 88.8 % -99.7 % and 94% -99.3%, respectively. Amino acid homology with LaSota,Clone30,F48E9 reference strains were 88.4 % -89.5 %,87.7 % -88.8 %,90.8 % -92.4 % , respectively.HN gene length of five NDV isolates was 1801 bp, encoding 571 amino acid, and contain 13 conserved C residue. Amino acid homology of 5 isolates with LaSota was 87.2 % -88.8 % , compared to 89.8 % -98.6 % homology with 8 strains from Shandong.%选取山东2007 ～ 2008年的5株NDV流行株,克隆融合蛋白(F)和血凝素-神经氨酸酶(HN)基因全长并测序.结果表明,5个分离株F基因长度为1662 bp,编码553个氨基酸.F蛋白裂解位点的氨基酸序列均为112 R-R-Q-K-R-F117,符合强毒株F基因裂解位点的氨基酸序列特征.对F基因47 ～420 nt序列进行比对,发现4株鸡源毒株基因型为Ⅶd,另一鸽源毒株基因型为Ⅵ.5个分离株间F基因核苷酸和氨基酸序列同源性分别为88.8％～99.7％和94％～99.3％,与LaSota、Clone30、F48E9的氨基酸同源性分别为88.4％～89.5％、87.7％～88.8％、90.8％～92.4％.HN基因长度为1801bp,编码571个氨基酸,5个毒株均含13个半胱氨酸残基且位置保守.与LaSota的氨基酸同源性为87.2％～88.8％,与8个山东NDV流行株的同源性为89.8％～98.6 ％.