混合黑穗病孢子侵染甘蔗品种NCO376的研究

摘要

In this study , NCO376 immunity to the sugar cane smut race 1 and 2 was used as host material, and smut spores were collected in National Nursery of Sugarcane Germplasm Resources( NNSGR) to study the resistance for NCO376 and amut physiological race in National Nursery of Sugancane (Germplasm Resources according to dipping, cutting the stems and shake off the Uatilago scitaminea spores, irrigating bactena liquid and other methods of artificial inoculation for sugarcane smut fungua infection experiments. The results showed that : in 5 × 106 spores/mL dipping group tear , the contol rate was 16 ％ , the lowest bacteria liquid infection rate was 2. 5 ％ , the highest cutting the stem infection rate was 25. 8 ％ ; in water dipping group test . bacteria liquid infection rate Was 0 , the cutting atem infection rate was 13 ％ , ahaking smut spores infection was 0. In the bacteria liquid dipping material group, the cutting stem group infection rate ＞ shake amut apores group ＞ dipping control group ＞ bacteria liquid irrigation group : in water dipping group test , the higher infection nue ior cutting stem was observed. and bacteria liquid irrigation and shake the smut spores was no easy to infect the host material. According to the standard dipping group control test , a new physiological rsce could existed in the National Nursery of Sugarcane Cermplasm Resources.%本研究利用对甘蔗黑穗病生理小种1和生理小种2免疫的甘蔗品种NCO376作为寄主材料,在云南开远蔗区搜集黑穗病孢子,依照浸渍、割苗涂抹、抖黑粉孢子、菌液浇灌等人工接种的方法进行甘蔗黑穗病菌的侵染实验,针对NCO376的抗性和黑穗病生理小种进行研究.结果表明,以5×106个孢子/mL的浸渍组试验中对照的侵染率为16%,割苗涂抹侵染率为25.8%,侵染率最高,菌液浇灌侵染率最低为2.5%;清水浸渍组试验中菌液浇灌侵染率为0,割苗涂抹侵染率为13%,抖黑穗孢子侵染率为0.材料NCO376在浸渍组中,割茎涂抹处理侵染率＞抖黑穗病孢子处理＞对照＞菌液浇灌处理;清水浸渍组中,割茎侵染率较高,菌液浇灌和抖黑穗病孢子相对不容易侵染宿主材料.依据标准的浸渍组对照试验,云南开远蔗区可能有新的生理小种存在.