几种抗冻剂对达氏鲟精子活力及运动时间的影响

摘要

[目的]为了研究不同浓度抗冻剂对达氏鲟精子活力、运动时间和寿命的影响,[方法]用0.7％氯化钠+0.02％氯化钾+3.5％葡萄糖的稀释液分别配制成4％甲醇(Methanol)、8％乙二醇(Ethylene Glycol)、12％1,3-丙二醇(1,3-Propanediol)、16％丙三醇(Glycerol)、20％二甲基甲酰胺(N,N-Dimethylformamide)和24％二甲亚砜(Dimethyl Sulfoxide)的6种抗冻剂,在4℃保存达氏鲟精子16 h后进行激活处理.[结果]结果表明:丙三醇、二甲基甲酰胺和二甲亚砜在浓度4％～24％时精子激活后有活力和寿命;甲醇、乙二醇和1,3-丙二醇在浓度4％ ～16％时精子激活后有活力和寿命,当浓度为20％和24％时精子全部死亡.甲醇、乙二醇、1,3-丙二醇、丙三醇、二甲基甲酰胺和二甲亚砜浓度分别为8％、8％、12％、4％、4％和8％时,最高精子活力分别为81％、32％、76％、64％、72％和67％,最长精子激烈运动时间和寿命分别为42和205 s、16和123 s、36和127 s、25和96 s、27和132 s、25和103 s.甲醇与其它几种抗冻剂相比,在8％甲醇中达氏鲟精子的激烈运动时间和寿命最长,分别为42和205 s.[结论]6种抗冻剂对达氏鲟精子有不同的毒性作用,保存后精子活力和寿命均降低.%[Objective] The present paper was to study the effects of different concentrations of cryoprotectants on the sperm motility,life-span and movement time of Acipenser dabryanus.[Method] Six kinds cryoprotectants of 4 ％ Methanol,8 ％ Ethyleneglycol,12 ％ Propanediol,6 ％ Glycerol,20 ％ N,N-Dimethylformamide and 24 ％ Dimethyl sulfoxide were respectively conducted with sperm diluents (0.7 ％ NaC1 + 0.02 ％ KC1 + 3.5 ％ Glu),and the sperm by cryoprotectants was preserved 16 hours at 4 ℃ and activated.[Result] The sperm of Acipenser dabryanus had sperm motility and life-span with 4 ％-24 ％ cryoprotectant (Glycerol,N,N-Dimethylformamide and Dimethyl Sulfoxide)and 4 ％-16 ％ cryoprotectant(Methanol,Ethyleneglycol and Propanediol),but the all sperm was died with 20 ％-24 ％ cryoprotectant (Methanol,Ethyleneglycol and Propanediol).The sperm motility was 82 ％,32 ％,76 ％,64 ％,72 ％ and 67 ％,respectively when 8 ％ Methanol,8 ％ Ethyleneglycol,12 ％ Propanediol,4 ％ Glycerol,4 ％ N,N-Dimethylformamide and 8 ％ Dimethyl suffoxide were applied,The drastic movement time and life-span of the sperm were the longest and respectively achieved 42 and 205 s,16 and 123 s,36 and 127 s,25 and 96 s,27 and 132 s,25 and 103 s.As compared with other cryoprotectants,the drastic movement time and life-span of the sperm were the longest and respectively achieved 42 and 205 s with 8 ％ Methanol.[Conclusion] Six kinds of cryoprotectants could produce toxicity on the sperm,and the motility and life-span of the sperm could be reduced after preserved by these cryoprotectants.