应用正交试验优化猪细小病毒N株在IBRS-2细胞转瓶培养

摘要

利用正交试验对猪细小病毒(PPV)N株在IBRS-2细胞转瓶培养中的培养条件进行优化,为PPVN株弱毒疫苗的大量培养工艺提供参考.设细胞培养液pH值(7.0、7.2和7.4)、接毒时间(0、24和48 h)、接毒剂量(0.10％、1.00％和10.00％)、收毒时间(48、72和96 h)等因子和水平.结果表明,PPV N株转瓶培养的最佳培养条件组合为细胞培养液pH值7.2,采用同步接毒,接毒剂量为0.10％,收毒时间为接毒后96 h.其中收毒时间是影响PPV N株毒价的最主要因素.%The orthogonal design was used to optimize the culture conditions,of PPV N strain in IBRS-2 cell cultivated in roller bottles to provide reference for the mass culture of PPV N attenuated vaccine.Affected factors were as follows:the pH value (7.0,7.2,7.4),the time of inoculation (0,24,48h),the dose of toxin (0.10 ％,1.00 ％,10.00 ％) and the time of harvesting (48,72,96 h).Results showed that the best culture conditions of PPV N strain IBRS-2 cells cultivated in roller bottles were as follows:pH =7.2,synchronization inoculation,0.10 ％ inoculation dose,harvesting the virus solution 96 h after inoculation.Virus solution harvest time is the most important factor affecting the virus titer of PPV N strain.