饥饿对卵形鲳鲹幼鱼不同组织抗氧化能力、Na+/K+-ATP酶活力和鱼体生化组成的影响

摘要

We measured the total anti-oxidation capability ( T-AOC) , superoxide dismutase ( SOD ) activities, malondialdehyde ( MDA) levels, Na +/K +-ATPase activity and biochemical composition in the branchial filament, liver and muscle of Trachinolus ovalus after starvation. The results show that the T-AOC increased in the branchial filament and muscle after 7-day starvation, whereas significantly decreased in the liver (P <0. 01). The SOD activities increased significantly in the branchial filament and muscle (P <0. 01) , but had no significant difference in the liver (P > 0. 05). The MDA levels in the branchial filament, liver and muscle increased after starvation, especially very significantly in the branchial filament (P <0. 01) , significantly in the liver (P <0. 05) , but not significantly in the muscle ( P > 0. 05 ). The Na +/K +-ATPase activity increased significantly in the branchial filament ( P < 0. 01) and in the muscle ( P < 0. 05) but decreased significantly in the liver ( P <0. 01) after 8-day starvation. The moisture content in the whole body of juvenile T. Ovatus increased while the crude fat, crude protein and energy values decreased greatly during starvation; and the relative loss rate of crude fat was higher than that of crude protein, indicating that juvenile T. Ovatus utilizes fat as energy source in early starvation, and with the extension of starvation, the protein will be consumed.%卵形鲳鲹(Trachinotus ovatus)幼鱼经短期饥饿处理后测定其鳃、肝脏和肌肉中的总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活力、丙二醛(MDA)质量摩尔浓度、Na+/K+-ATP酶活力和全鱼生化组成.结果显示,饥饿7d后鳃丝和肌肉的T-AOC显著升高,肝脏的T-AOC显著下降(P＜0.0);鳃丝和肌肉SOD活力均显著升高(P＜0.01),肝脏的SOD活力变化不显著(P＞0.05);鳃丝、肝脏和肌肉的b(MDA)在饥饿后上升,鳃丝b(MDA)与对照组差异极显著(P＜0.0),肝脏b(MDA)与对照组相比差异显著(P＜0.05),肌肉b(MDA)差异不显著(P＞0.05).饥饿8d后鳃丝Na+/K+-ATP酶活力显著升高(P＜0.01),肝脏Na+/K+-ATP酶活力显著下降(P＜0.01),肌肉Na+/K+-ATP酶活力也有所升高(P＞0.05);饥饿后鱼体水分质量分数上升,粗蛋白质量分数、粗脂肪质量分数和能值下降,而且相对损失率是粗脂肪＞粗蛋白,表明卵形鲳鲹幼鱼在饥饿早期主要消耗脂肪向机体提供能量,随着饥饿时间的延长,蛋白质将被消耗.