维生素E和裂壶藻对斑节对虾精荚再生的协同作用

摘要

文章探究了维生素E(Vitamin E,VE)和裂壶藻(Schizochytrium sp.,Sch)对斑节对虾(Penaeus monodon)雄虾精荚再生的作用.以基础饲料配制了4种不同配合饲料(0添加组;VE200组;1% Sch组;VE200+1% Sch组),饲喂被挤压排出精荚的成熟雄性斑节对虾40 d,评估斑节对虾雄虾精荚再生后的精子数量和质量.结果表明,添加量为200 mg·kg–1VE饲料组的活精子数百分比显著高于对照组(P<0.05);单一添加1%裂壶藻饲料组的活精子数与对照组间差异不显著(P>0.05);共同添加200 mg·kg–1VE和1%裂壶藻的饲料组的精子总数显著高于对照组(P<0.05).综合精荚质量、精子数量及活精子数百分比3个指标得出,饲料中共同添加1%裂壶藻与200 mg·kg–1VE对斑节对虾雄虾精荚的再生和提高精子数量具有协同促进作用.%To investigate the effects of Vitamin E and Schizochytrium sp.on the spermatophore regeneration in Penaeus monodon, we designed four different contents of Vitamin E and Schizochytrium sp.in the basal diet(control group;VE200;1% Schizochytri-um sp.;VE200+1% Schizochytrium sp.)to feed mature male P.monodon which had been extruded spermatophore for 40 d.The res-ults show that the percentage of viable spermatozoa added in 200 mg·kg–1Vitamin E diet group was significantly higher than that in the control group(P<0.05).The sperm quantity of single added in 1% Schizochytrium sp.diet group was not significantly different with control group(P>0.05).The total number of spermatozoa fed with 200 mg·kg–1of Vitamin E and 1% Schizochytrium sp.was significantly higher than that of the control group (P<0.05). According to the spermatophore quality, sperm quantity and living sperm percentage,adding 200 mg·kg–1of Vitamin E and 1% Schizochytrium sp.in feed can promote the spermatophore regeneration in the male P.monodon and improve the sperm quantity effectively.