2株溶磷、解钾与产IAA的内生真菌菌株的筛选、鉴定及促生作用研究

摘要

Phosphate/potassium(K/P)-solubilizing and IAA-secreting strains of endophytic fungi were isolated from dicranopteris dichotoma as part of preponderant ferns growing in potassium deposit.And their growth promoting function on crops was researched.K/P-solubilizing endophytic fungi were isolated and identified using morphological and cultural characteristics and 5.8S,18S rDNA ITS sequence analysis.The K/P-solubilizing capacity of strains were measured by Petri dishes and broth medium experiments.The growth promoting effect of strains on corn seedling was studied using tieback technology and soil pot experiment.Result showed that 42 strains of endophytic fungi were screened from dicranopteris dichotoma.There were two stains(MQ013,MQ039)among them with the ability of dissolving P/K and secreting IAA.Strain MQ013 was identified as Aspergillus awamori,and MQ039 as Aspergillus niger.The experiment of plant growth promoting function showed that strains MQ013,MQ039 effectively improved the content of chlorophil,potassium and phosphate in body of corn plant.It was concluded that MQ013 and MQ039 have a certain capacity of solubilizing P/K and secreting IAA,and have a significant growth promoting effect on corn seedling.%从钾矿区优势蕨类植物-芒萁内生真菌中筛选出具溶磷、解钾、分泌吲哚乙酸(IAA)的功能菌株,并研究其对农作物的促生作用.采用菌株的形态学特性、培养特征及5.8S rDNA、18S rDNA ITS序列分析方法对菌株进行鉴定,并结合液体培养和固体培养方法初步测定菌株的溶磷、解钾能力,采用回接及盆栽试验研究它们对玉米幼苗的促生作用.从42株芒萁内生真菌中筛选得到2株具有高效溶磷、解钾、分泌IAA功能的内生真菌菌株(编号为MQ013和MQ039),经鉴定MQ013菌株为泡盛曲霉(Aspergillus awamori),MQ039菌株为黑曲霉(Aspergillus niger).促生作用试验结果显示,MQ013、MQ039菌株能有效提高玉米植株体内叶绿素及磷、钾含量.MQ013、MQ039菌株具有一定的溶磷、解钾和分泌IAA活性,并对玉米幼苗的生长有明显的促进作用,该菌株在研制高效生物肥料接种剂方面可能具有较大潜力.