基于31P核磁共振分析的植物体内磷分级研究

摘要

为了深入了解植株残体中磷的分级情况以及在降解过程中的供磷情况,采用31P核磁共振(31 phosphorus nuclear magnetic resonance,31P-NMR)技术,分析了油菜、蚕豆不同部位的磷的分级情况,并采用室内模拟的方法评价不同植物材料还田后的有效性.结果表明,不同植物材料中都以正磷酸盐所占比例最高,在60%~67%之间,其次为磷酸单酯,占浓缩液全磷的比例为20%~28%;高磷植株的正磷酸盐和磷酸单酯含量显著高于中磷和低磷植株,这与植物吸收磷的特性以及磷在植物体内的储存方式有关.结果表明,31P-NMR分析技术能较好地反应植物材料还田后的供磷潜力.%Phosphorus(P)mineralization from crop residues is usually predicted from total P or carbon:phosphorus(C∶P)ratios.However,these measures have limited accuracy as they do not take into account the presence of different P forms that may be mineralised at different rates.In this study P form in Brassica campestris L.and Vicia faba L.was determined using solution31P nuclear magnetic resonance(NMR)spectroscopy to understand the potential fate of residue P in soils.The results showed that the main forms of P detected were orthophosphate(60%~67%),phosphomonoester(20%~28%)and the two forms detected in plants with higher P content were higher than the plants with less P content and this might contributed to the character of P uptake and accumulation in plants.The results showed that 31P nuclear magnetic resonance played an important role in plant phosphorus speciation.