产超广谱酶大肠埃希菌和肺炎克雷伯菌对头孢替坦等抗菌药物的耐药性研究

摘要

目的 初步探讨头孢替坦等8种临床常用抗菌药物对产超广谱酶( ESB Ls)的大肠埃希菌及肺炎克雷伯菌的体外抗菌活性,为临床合理用药提供依据.方法 四川大学华西医院抗感染药物研究室分离自临床感染患者的120株经双纸片法确证为产ESBLs大肠埃希菌及肺炎克雷伯菌,采用琼脂二倍稀释法进行8种临床常用抗菌药物体外抗菌活性试验.结果 亚胺培南等碳青霉烯类抗生素对产ESBLs抗菌作用有较强的抗菌活性,大肠埃希菌的MIC50为＜0.03μg/ml,MIC90为0.06 μg/ml,肺炎克雷伯菌的MIC50为＜0.03 μg/ml,MIC90为0.06 μg/ml；头孢替坦对大肠埃希菌及肺炎克雷伯菌也有很好的抗菌活性,MIC50分别为0.06和0.06 μg/ml,MIC90分别为1和2μg/ml.结论头孢替坦对临床常见产ESBLs的大肠埃希菌和肺炎克雷伯菌具有良好的抗菌活性.%Objective To determine the vitro activity of the 8 antibiotics including cefotetan against clinical isolates of extended spectrum beta-lactamases(ESBLs) of Escherichia coli and klebsiella peneumoniae and provide experimental evidences for rational use of antibiotics . Methods A total of 120 strains of acinetobacter baumannii were isolated from infect room of west china hospital . The MIC of 8 antibiotics were evaluated by double-agar dilution method. Results Among the 120 isolatess, the resistance rates to impenen is better than other antibiotics, MIC^ of Escherichia coli was <0. 03|xg/ml, MIC^, was 0. 06(jLg/ml; MICjo of Klebsiella Peneumoniae was <0.03(Ag/ml, MIC,,, was 0.06fj￡/ml. Cefotetan was similar to impenen, MIC^ of Escherichia coli and klebsiella peneumoniae were 0.06(jLg/ml and 0.06ng/ml, MIC,,, were 1 fig/ml and 4ng/ml. Conclusion Cefotetan is a good antibiotic activity for the treatment to ESBLs of Escherichia coli and klebsiella peneumoniae infections.