Objective Establishing the culture of human normal melanocytes in vitro, and studying effects of Interleukin-6 on cultured human melanocytes. Methods MTT method, NaOH 2 assay were employed to measure the cell viability, melanin synthesis of melanocytes, apoptosis rates of melanocytes were detected by using flow cytometer. Results Interleukin-6 induced a mild effect on melanocytic viability, while it showed a significant decrease in the cell counts, melanin synthesis and cell apoptosis. Conclusion Interleukin-6 can inhibit melanocytic proliferation and melanin synthesis. It is worth to study later. It makes valid to make use of Interleukin-6 for treatment of the pigmented skin diseases.%目的 建立正常人表皮黑素细胞体外培养体系,研究白介素-6(Interleukin-6,IL-6)对体外培养的黑素细胞的影响.方法 采用四甲基偶氮唑盐(MTT)法测定白介素-6对黑素细胞活力的影响,NaOH裂解法测定黑素合成,流式细胞仪检测黑素细胞的凋亡率.结果 白介素-6对黑素细胞活力有抑制作用,能使细胞增殖力降低,黑素合成减少,增加黑素细胞的凋亡率.结论 白介素-6对体外培养的黑素细胞增殖及黑素生成都有抑制作用,这为临床应用白介素-6治疗色素性疾病提供了实验依据.
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