Method for in vitro cultivation of mammalian ovarian follicles and determining effects of chemical or physical influences on folliculogenesis

摘要

The present invention is related to a method for in vitro culture of mammalian ovarian follicles, comprising the following subsequent steps:providing a suitable container for the in vitro culture,selecting a follicle from an ovary of a mammal, said follicle comprising at least a theca or pre-theca cell, a granulosa cell and an oocyte,an optional first culture step using an attachment prohibiting first medium free from oil,a second culture step using an attachment promoting second medium free from oil arranged to, andthe retrieval of the matured follicle.