藿莲香Ⅱ号复方的致突变性研究

摘要

Objective To evaluate the toxicological effect of Huo lianxiang Ⅱ according to Food Saf ety and Toxicology Evaluation Procedures and Methods (GB15193-2003) .Methods Acute oral toxicity test ,marrow cell micronucleus test PCE ,marrow chromosomal aberration test of mice and Ames were tested .Results Mice oral M TD is 10 g/kgBW .Micronucleus test of bone marrow PCE cell in mice ,chromosomal aberration test in mice bone marrow and Ames test results were negative .Conclusion Huo lianxiang Ⅱ is of no mutagenic effect .%目的：根据《食品安全性毒理学评价程序和方法》（GB15193-2003）对中药复方藿莲香Ⅱ号进行相关的毒理学评价。方法应用急性毒性试验、小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验和 Ames 试验检测该复方的急性毒性和致突变性。结果急性经口毒性试验：雌性、雄性小鼠经口最大无毒浓度（M TD）为10 g/kg 体质量，属实际无毒；致突变实验：小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验和 Ames 试验结果均为阴性，显示在本实验条件下，该配伍未见有致突变性作用。结论藿莲香Ⅱ号未见有致突变性作用。