青藤碱对佐剂性关节炎大鼠Toll 样受体2 mRNA 及 MyD88 mRNA 表达的影响

摘要

Objective To investigate the effects of sinomenine on the expression of TLR2 mRNA and MyD88 mRNA in rats with adjuvant arthritis .Methods Forty SD rats were randomly divided into model group , sinomenine group ,methotrexate group and normal control group .Rats from the first three groups were induced rat model of adjuvant arthritis by intradermal injection of Freund′s in right paw ,and rats from the normal control group were injected saline .After the rats from different groups were treated for four weeks respectively ,the rats knee synovium were taken out to detect the expression of TLR2 mRNA and MyD88 mRNA using real-time PCR method ,and the expression of inflammatory cytokines TNF-α by ELISA.Results The expression of TLR2 mR-NA ,MyD88 mRNA and TNF-αof synovial tissue in the model rats significantly higher than those in the normal control group .The difference was statistically significant (all P<0 .01) .After treated by sinomenine ,the expres-sion of TLR2mRNA ,MyD88 mRNA and TNF-αof synovial tissue significantly decreased compared with the mod-el rats ,and the difference was statistically significant (all P<0 .01) .Conclusion Sinomenine can improve arthritis in rats with adjuvant arthritis ,which may be related to inhibition of TLR2 signaling pathway.%目的：探讨青藤碱对佐剂性关节炎大鼠Toll样受体（TLR）2mRNA及MyD88mRNA表达的影响。方法清洁级SD大鼠40只，随机分为模型组、青藤碱组、甲氨蝶呤组及正常对照组。前3组大鼠右后足跖皮内注射弗氏完全佐剂诱发大鼠佐剂性关节炎模型，正常对照组大鼠右后足跖皮内注射等量0.9％氯化钠注射液。各组分别干预4周后，取膝关节滑膜，采用实时聚合酶链反应（PCR）法检测TLR2mRNA，MyD88mRNA表达，酶联免疫吸附试验（ELISA）法检测肿瘤坏死因子（TNF）-α。结果模型组大鼠膝关节滑膜组织TLR2mRNA，MyD88mRNA表达及TNF-α均较正常对照组明显升高，差异有统计学意义（均为P＜0．01）。经青藤碱治疗后，TLR2mRNA，MyD88mRNA表达及TNF-α均较模型组明显下降，差异有统计学意义（均为P＜0．01）。结论青藤碱可改善佐剂性关节炎大鼠的关节炎症，该作用可能与抑制TLR2通路有关。