电针足三里对应激性胃溃疡大鼠胃组织 HSP70mRNA 的影响

摘要

目的：观察电针足三里对应激性胃溃疡大鼠胃组织中热休克蛋白70(Heat Shock Protein 70,HSP70) mRNA 表达的影响,探讨电针足三里防治胃溃疡的分子机制。方法将51只 SD 大鼠随机分为正常对照组(正常组)、应激性胃溃疡组(模型组)和电针足三里组(电针组)。采用冷-束缚法制作应激性胃溃疡模型。造模后次日起电针组每天电针足三里20 min,连续3 d,左右足三里穴交替进行；模型组只进行与电针组一样的固定处理；正常组不做任何处理。按 GUTH’s 法计算胃黏膜损伤指数(Ulcer Index,UI)、HE 染色法观察胃组织病理形态、RT-PCR 法检测胃组织 HSP70 mRNA 表达。结果模型组与正常组比较,胃黏膜上可见数量、大小不等的明显溃疡,UI 显著升高(P＜0.001),HSP70 mRNA 表达显著降低(P＜0.05)；电针组与模型组比较,胃组织病理变化有所改善,胃黏膜 UI 显著降低(P＜0.05),HSP70 mRNA 表达显著升高(P＜0.01)。结论电针足三里可以上调应激性胃溃疡大鼠胃组织 HSP70 mRNA 表达,促进胃黏膜损伤的修复。%Objective To investigate the effect of electroacupuncture at point Zusanli (ST36) on gastric expression of heat shock protein 70 (HSP70) mRNA in rats with stress gastric ulcer and explore the molecular mechanism of its prevention and treatment of gastric ulcer. Method Fifty-one SD rats were randomly allocated to normal control (normal), stress gastric ulcer (model) and electroacupuncture at point Zusanli (electroacupuncture) groups. A model of stress gastric ulcer was made by cold restraint. From the second day after model making, the electroacupuncture group received electroacupuncture at point Zusanli 20 min once daily for three consecutive days, with left and right points Zusanli alternately needled; the model group, the same restraint treatment as in the electroacupuncture group; the normal group, no treatment. Gastric mucosal ulcer index (UI) was calculated using the GUTH’s method. The pathological morphology of gastric tissue was observed by HE staining. Gastric HSP70 mRNA expression was determined by RT-PCR. Result Different numbers and sizes of obvious gastric mucosal ulcers were observed, UI increased significantly (P<0.001) and HSP70 mRNA expression decreased significantly (P<0.05) in the model group compared with the normal group. The pathological change in gastric tissue improved somewhat, gastric mucosal UI decreased significantly (P<0.05) and HSP70 mRNA expression increased significantly (P<0.01) in the electroacupuncture group compared with the model group. Conclusion Electroacupuncture at point Zusanli can upregulate gastric HSP70 mRNA expression and promote the repair of gastric mucosa injuries in rats with stress gastric ulcer.