十全大补膏中芍药苷的含量测定

摘要

A HPLC method for the determination of paeoniflorin in tonic semifluid extract of ten ingredients was established. The HPLC analysis was performed on Kromasil C18column(4.6 mm×250 mm,5 μm),acetonitrile-water (17 : 83,V/V)as the mobile phase,and the column temperature was 30 ℃,injection volume was 10 μL,the detective wavelength was at 230 nm,at a flow rate of 1.0 mL/min. Results revealed that paeoniflorin at the content of 0.083 6~0.627 0 μg showed good linearity(r= 0.999 8),the recovery rate was 98.98%~100.47%,RSD was 0.65%(n=6). This method is highly specific,sensitive,reproducible,and simple,which can be used for the quality control of this preparation.%建立了高效液相色谱法测定十全大补膏中芍药苷含量的方法.采用Kromasil C18色谱柱(4.6 mm×250 mm,5 μm),以乙腈-水(17 : 83,V/V)为流动相,柱温30 ℃,进样量10 μL,检测波长230 nm,流速1.0 mL/min.结果表明,芍药苷在0.0836~0.6270 μg范围内线性关系良好(r= 0.9998),加样回收率为98.98%~100.47%,相对标准偏差为0.65%(n=6).本方法专属性强、灵敏度高、重现性好、操作简便,适用于十全大补膏的质量控制.