慢性阻塞性肺疾病患者诱导痰中DCs及CCR6的变化及意义

摘要

目的 分析树突状细胞(DCs)及趋化因子受体6(CCR6)在慢性阻塞性肺疾病(COPD)发病机制中的作用.方法 以COPD患者为研究对象,并以健康人作为对照,采用沙丁胺醇加高渗盐水超声雾化吸入方法诱导排痰,以流式细胞技术检测痰液中DCs水平,以ELISA检测CCR6的含量.同时,常规方法测定肺功能FEV1％及FEV1/FVC.结果 DCs、CCR6在COPD分期的GOLD Ⅰ级、GOLDⅡ级以及GOLDⅢ～Ⅳ级均高于正常对照组和吸烟无COPD组(P＜0.01).DCs、CCR6与COPD患者FEV1％之间均呈负相关关系(P＜0.05或＜0.01).结论 COPD患者DCs及CCR6升高,且与病情的严重程度明显相关,提示DCs及CCR6可能参与了COPD的炎症反应过程.%Objective To investigate the function of dendritic cells ( DCs) and chemkine receptor 6 ( CCR6) in the pathogenesis of chronic obstructive pulmonary disease (COPD). Methods The cells count determined by DCs were analyzed with flow cytometry in the sputum collected from COPD groups, cigarette smoke group and control group. CCR6 was analyzed by ELISA method. FEV1% and FEV1/FVC were determined. Results DCs and CCR6 in the GOLD I -IV of COPD groups showed upregulation compared with those in cigarette smoke group and control group. DCs and CCR6 were negative correlation with FEV1% in COPD group (P<0.05 or <0.01). Conclusion DCs and CCR6 maybe contribute to the inflammatory reaction of COPD.