食管癌TDLN细胞对食管癌裸鼠移植瘤的体内抑瘤作用

摘要

目的 探讨肿瘤引流区淋巴结细胞对食管癌裸鼠移植瘤的体内杀伤作用,为其临床应用提供依据.方法 将30只食管癌移植瘤裸鼠模型随机分为对照组、IL-2组、LAK组、I-TDLN组及G-TDLN组.分别予生理盐水、IL-2、LAK细胞及体外培养的I-TDLN、G-TDLN细胞局部注射干预.分别于干预后1、2、3、4周测定各组肿瘤抑制率;干预后4周测定各组肿瘤细胞凋亡率;观察肿瘤组织病理学变化.结果 TDLN组各时点肿瘤抑制率均明显高于对照组、IL-2组及LAK组,G-TDLN组高于I-TDLN组(P均＜0.05),各组肿瘤抑制率均逐渐升高,于第3周达高峰;对照组、IL-2组、LAK组、I-TDLN组、G-TDLN组细胞凋亡率依次升高,P均＜0.05.LAK组及TDLN组肿瘤组织均有T淋巴细胞和DC浸润,但TDLN组明显多于LAK组.结论 体外培养的食管癌TDLN细胞在体内可抑制食管癌移植瘤生长,其机制为直接杀伤肿瘤细胞并诱导肿瘤细胞凋亡.本研究为食管癌TDLN细胞用于临床食管癌的细胞免疫治疗提供了理论依据.%Objective To study the in vivo tumor-inhibition effects of tumor-draining lymph node (TDLN) on tumorbaring nude mice, so as to provide basis for its clinical application. Methods Thirty tumor-baring nude mice model were randomized into control group, IL-2 group , LAK group, I-TDLN group and G-TDLN group. Then were injected with NS,IL-2 , LAK cell and I-TDLN, G-TDLN cell cultured in vitro, respectively. The tumor inhibition ratio (TIR) was evaluated 1,2,3,4 week after injection;the apoptosis ratio of tumor cell was measured in the 4th week;the histopathological changes of tumor were observed. Results The TIR of I-TDLN group was significantly greater than that in LAK group,IL-2 group and control group, but which of the G-TDLN group was greater than that of I-TDLN group( P ＜0.05). The TIR of each group increased gradually until the 3th week. The apoptosis ratio of the control group, IL-2 group, LAK group, I-TDLN group, G-TDLN group increased gradually ( all P ＜ 0.05). T lymphocyte and DC infiltration were found in the LAK group and TDLN group, and which was even apparent in TDLN group. Conclusions TDLN cells cultured in vitro can inhibit the growth of transplantable tumor of esophageal carcinoma, the mechanism maybe kill tumor cells and induce its apoptosis.This study provides theoretical basis for the cellular immunotherapy on esophageal carcinoma by TDLN cell.