舒芬太尼预先给药对脑缺血再灌注损伤大鼠海马CA1区形态学和脑组织总钙的影响

摘要

目的 观察舒芬太尼预先给药对全脑缺血再灌注损伤大鼠海马CA1区形态学和脑组织总钙的影响.方法 70只雄性SD大鼠随机分为5组,每组14只,假手术组(S组)、缺血再灌注组(I/R组)和舒芬太尼不同剂量预先给药组(L组、M组、H组).缺血再灌注组和舒芬太尼不同剂量预先给药组分别在缺血前泵入生理盐水和0.5、1.5和4.5 μg/kg舒芬太尼,持续泵注5 min,暂停5 min,重复3次(容积1 mL/5 min,总时间30 min).采用四动脉阻断法(pulsinelli-4vo法)致全脑缺血,15 min后恢复再灌注.再灌注24 h后每组随机选6只,光镜和电镜下观察海马CAl区形态学变化,其余8只断头取脑,测脑组织总钙含量.结果 与S组比较,I/R组、L组、M组和H组海马CA1区损伤加重,病理学分级增加(P＜0.05),脑组织总钙含量增加(P＜0.05)；与I/R组比较,M组海马CA1区损伤减轻,病理学分级降低(P＜0.05),L组、M组和H组脑组织总钙含量降低(P＜0.05)；与M组比较,L组和H组病理学分级无统计学意义(P＞0.05),L组脑组织总钙增高(P＜0.05),H组无统计学意义(P＞0.05).海马CA1区透视电镜:舒芬太尼预先给药各组核膜、粗面内质、突触等均较I/R组损伤减轻.结论 舒芬太尼预先给药可以减轻海马CA1区的损伤,降低缺血再灌注损伤所致脑组织总钙含量,以1.5 μg,/kg的作用最显著.%Objective To investigate the effects of sufentanil pretreatment on hippocampus CA1 and total calcium of brain tissue in rats with cerebral ischemia-reperfusion. Methods Seventy male SD rats were randomly divided into five groups; sham group (group S) , ischemia-reperfusion group (group I/R) and different concentrations of sufentanil pretreatment groups (group L, M, H). The rats in group I/R and sufentanil pretreatment groups received normal saline and sufentanil infusion intravenously at 0. 5, 1.5 and 4.5 μg/kg in the regime (3×5 min infusion with 5 min interval) before cerebral ischemia. Four-vessel occlusion was used to establish the global cerebral ischemia reperfusion model in the study groups. An acute experimental cerebral ischemia rats model induced for 15 min, 24 hours after reperfusion, randomly selected six rats to observe light microscope morphological changes of the hippocampus CA1, randomly selected two of the six rats to observe e-lectron microscopy changes of the hippocampus CA1 and the remaining eight rats to measure the total calcium in brain tissue in each group. Results Compared with group S, the hippocampus CA1 injuries, histological grades and the brain total calcium increased significantly in group I/R, L, M and H (P < 0.05) . Compared with group I/R, the hippocampus CA1 injuries and the histological grades decreased in group M (P < 0.05) , the brain total calcium decreased in group L, M and H (P < 0.05). Compared with group M, the histological grades were no significant in group L and group H, the brain total calcium increased in group L (P <0.05) , in group H were no significant (P < 0. 05). The hippocampus CA1 electron microscopy: group L, M and H were less injury than group I/R in nuclear membrane, rough endoplasmic retieula and synaptic.Conclusion Sufentanil pretreatment can protect rat brain against ischemia-reperfusion injury, especially 1.5 μg/kg.