舒芬太尼预先给药对大鼠急性局灶性脑缺血-再灌注损伤的保护作用

摘要

目的 观察舒芬太尼预先给药对大鼠大脑中动脉栓塞(MCAO)所致局灶性脑缺血-再灌注损伤的保护作用.方法 40只雄性大鼠随机均分为四组,分别于缺血造模前腹腔注射舒芬太尼3μg/kg(S_1组)、6μg/kg(S_2组)、9μg/kg(S_3组)和等容积生理盐水为对照组(C组).给药后30 min,所有动物用右侧颈内动脉尼龙线线栓法致MCAO,120 min后恢复再灌注.再灌注24 h后记录神经功能障碍评分(NDS).随后取大脑切片行TTC染色,计算脑梗死容积百分比.结果 再灌注后24h,S_1组NDS评分明显高于其他三组(P<0.05),梗死容积百分比明显低于其他三组(P<0.05),S_2、S_3、C组间NDS评分和梗死容积百分比差异均无统计学意义.结论 舒芬太尼3μg/kg预先给药可明显减轻大鼠局灶性脑缺血-再灌注损伤.%Objective To investigate whether sufentanil pre-dosage induces protection aganist acute focal cerebral ischemia-reperfusion injury in rats.Methods Forty male SD rats were randomly divided into four groups with 10 rats each.Before establishing animal model,the rats were intraperitoneally injected sufentanil 3μg／kg(group S_1)，6μg/kg(group S_2)，9μg/kg(group S_3)or normal saline(group C)．At 30 min after injection,all rats subjected to the right middle cerebral artery occlusion(MCAO)for 120 min,which was followed by reperfttsion. The neurological deficit scores(NDS)were evaluated at 24 h after reperfttsion.Infarct volume,as a percentage of volume at normal cerebral hemisphere,was determined by TTC staining.Results At 24 h after reperfusion,the NDS was higher and infarct volume was less in group S_1 than those in groups of S_2,S_3 and C(P<0.05).There were no significant differences in NDS and infarct volume among groups of S_2,S_3 and C. Conclusion Sufentanil preconditioning can protect rat brain against ischemiareperfusion injury.