Objective To investigate the relationship between methylation status of adenomatosis polyposis coli gene ( APC) promoter and environmental factors in the predisposition of prostate cancer (Pea) in Chinese. Methods Bisulphite sequencing PCR were used to detect the expression of APC genes in prostate cancer tissues from 60 patients and hy-perplastic tissues from 40 benign prostate hyperplasia ( BPH) patients. Environmental risk factors related with Pca were also collected including demographic information, body mass index (BMI) , drinking, tea drinking, smoking, sports. Relationship between methylation of APC gene, environmental risk factors and the PCa prevalence was analysised by using uni-variate and multivariate method. Results The methylation rates of CG sites in promoter of APC genes were 14. 00% in PCa and 1. 19% in BPH. Methylation rate in APC genes was associated with PSA, Gleason score, pathologic stage. PCa was correlated with drinking habit of wire (OR = 2.46) or tea ( OR = 0. 29). Conclusions The methylation of promoter in APC genes is related to the development of PCa and the classification of risk. Methylation of APC genes may serve as a promising method for early diagnosis of PCa. The drinking habit of wine or tea is also a risk factor for PCa.%目的 探讨腺瘤样结肠息肉易感基因(APC)启动子区CpG甲基化及环境危险因素与中国人群中前列腺癌(PCa)发病之间的关系.方法 收集60例PCa和40例前列腺增生(BPH)患者的组织标本.用亚硫酸盐修饰后测序法检测PCa组织及BPH组织中APC基因启动子区CpG甲基化情况.同时收集人口学资料以及体质量指数(BMI)、饮酒、饮茶、吸烟、体育运动等环境危险因素资料,用单因素和多因素分析法研究APC基因甲基化及环境危险因素与PCa患病之间的关系.结果 APC基因在PCa及BPH组织中CG位点甲基化率为14.00％、1.19％；基因甲基化率与前列腺特异性抗原(PSA)、Gleason评分、病理分期和PCa临床分期之间关系密切；PCa发病与饮酒、饮茶有关,比值比分别为2.46、0.29.结论 APC基因启动子区CpG甲基化与PCa发生及发展有关,其甲基化率的变化与PCa的临床分期关系密切.饮酒、饮茶是导致PCa的环境危险因素.
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